bisulfite dna pyrosequencing (at positions 318, 321, and 328) (Pyrosequencing Inc)
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Bisulfite Dna Pyrosequencing (At Positions 318, 321, And 328), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter"
Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter
Journal: MethodsX
doi: 10.1016/j.mex.2021.101445
Figure Legend Snippet: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High DNA marker (Biolabmix, Russia) with size in bp indicated.
Techniques Used: Sequencing, Methylation, Amplification, Marker
Figure Legend Snippet: Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing.
Techniques Used: Methylation
Figure Legend Snippet: Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.
Techniques Used: Methylation
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Methylation:Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter Article Snippet: Fig. 2 Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing. .. Fig 2 Fig. 3 Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by |