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bisulfite dna pyrosequencing (at positions 318, 321, and 328)  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc bisulfite dna pyrosequencing (at positions 318, 321, and 328)
    Method of targeted bisulfite massive parallel sequencing of the <t>human</t> <t>LINE-1</t> retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High <t>DNA</t> marker (Biolabmix, Russia) with size in bp indicated.
    Bisulfite Dna Pyrosequencing (At Positions 318, 321, And 328), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite+dna+pyrosequencing+(at+positions+318%2C+321%2C+and+328)/bisulfite+pyrosequencing/pmc08374674-80-27-29
    Average 90 stars, based on 1 article reviews
    bisulfite dna pyrosequencing (at positions 318, 321, and 328) - by Bioz Stars, 2026-11
    90/100 stars

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    1) Product Images from "Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter"

    Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter

    Journal: MethodsX

    doi: 10.1016/j.mex.2021.101445

    Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High DNA marker (Biolabmix, Russia) with size in bp indicated.
    Figure Legend Snippet: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High DNA marker (Biolabmix, Russia) with size in bp indicated.

    Techniques Used: Sequencing, Methylation, Amplification, Marker

    Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing.
    Figure Legend Snippet: Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing.

    Techniques Used: Methylation

    Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.
    Figure Legend Snippet: Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.

    Techniques Used: Methylation

    Related Articles

    Methylation:

    Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter
    Article Snippet: Fig. 2 Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing. .. Fig 2 Fig. 3 Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells. ..



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    Pyrosequencing Inc bisulfite dna pyrosequencing (at positions 318, 321, and 328)
    Method of targeted bisulfite massive parallel sequencing of the <t>human</t> <t>LINE-1</t> retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High <t>DNA</t> marker (Biolabmix, Russia) with size in bp indicated.
    Bisulfite Dna Pyrosequencing (At Positions 318, 321, And 328), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bisulfite+dna+pyrosequencing+(at+positions+318%2C+321%2C+and+328)/bisulfite+pyrosequencing/pmc08374674-80-27-29
    Average 90 stars, based on 1 article reviews
    bisulfite dna pyrosequencing (at positions 318, 321, and 328) - by Bioz Stars, 2026-11
    90/100 stars
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    Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High DNA marker (Biolabmix, Russia) with size in bp indicated.

    Journal: MethodsX

    Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter

    doi: 10.1016/j.mex.2021.101445

    Figure Lengend Snippet: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter. a. Schematic representation of the targeted bisulfite massive parallel sequencing of 19 CpG sites in the LINE-1 promoter. The red frame indicates the analyzed CpG sites in this study, and the blue frame indicates the most frequently analyzed CpG sites as determined using the Qiagen pyrosequencing LINE-1 kit (explanations are in the text). The schematic profile of the plot was obtained from the LINE-1 methylation index across the 5’-UTR region in analyzed samples. b. Gel image of the results of the amplified LINE-1 sequences for five samples after the first PCR with attached adapters and the same samples with attached indices after the second PCR. M represents Sky-High DNA marker (Biolabmix, Russia) with size in bp indicated.

    Article Snippet: Fig 2 Fig. 3 Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.

    Techniques: Sequencing, Methylation, Amplification, Marker

    Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing.

    Journal: MethodsX

    Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter

    doi: 10.1016/j.mex.2021.101445

    Figure Lengend Snippet: Relationship between the average LINE-1 methylation index (%) measured by the introduced method (NGS) and bisulfite DNA pyrosequencing.

    Article Snippet: Fig 2 Fig. 3 Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.

    Techniques: Methylation

    Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.

    Journal: MethodsX

    Article Title: Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter

    doi: 10.1016/j.mex.2021.101445

    Figure Lengend Snippet: Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.

    Article Snippet: Fig 2 Fig. 3 Relationship between methylation indices of individual CpG sites as measured by the introduced method and average LINE-1 methylation index (%) as assessed by bisulfite DNA pyrosequencing (at positions 318, 321, and 328) trophoblast and ACTA2+ cells.

    Techniques: Methylation